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human liver cancer stem cells  (Celprogen Inc)


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    Celprogen Inc human liver cancer stem cells
    Human Liver Cancer Stem Cells, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+liver+cancer+stem+cells/Human+Liver+Cancer+Stem+Cells/custom%4036116-43%4041772397
    Average 92 stars, based on 5 article reviews
    human liver cancer stem cells - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Modification:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc

    Transduction:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc

    Isolation:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc

    Stem Cell Culture:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc

    Cell Culture:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc

    Flow Cytometry:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc

    Cytometry:

    Article Title: Regulation of Autophagy and Metabolism in Hepatocellular Carcinoma: Involvement of Wnt‐β‐Catenin Pathway
    Article Snippet: h 10% Fetal Bovine Serum (HyClone) and antibiotics. Human liver cancer stem cells (CSCs) were isolated from primary tumours and cultured in stem cell culture medium as per the supplier's instructions (Celprogen, Torrance, CA). Human normal hepatocytes were purchased from ATCC. All cells were Mycoplasma free (as per detection kit from Lonza) and used within 3 months of continuous passage. The protoc



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    Image Search Results


    Levels of AKT signaling molecules (phosphorylated AKT and phosphorylated mTORC1) in normal hepatocytes and LCSCs under various exosomes. (CE: control cellular exosome, UPE: upregulated PRELI cellular exosome, DPEs: downregulated PRELI cellular exosome) (* p < 0.05, ** p < 0.01,*** p < 0.001).

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Profiling of PRELI-Modulated Exosomes and Effects on Hepatic Cancer Stem Cells

    doi: 10.3390/ijms252413299

    Figure Lengend Snippet: Levels of AKT signaling molecules (phosphorylated AKT and phosphorylated mTORC1) in normal hepatocytes and LCSCs under various exosomes. (CE: control cellular exosome, UPE: upregulated PRELI cellular exosome, DPEs: downregulated PRELI cellular exosome) (* p < 0.05, ** p < 0.01,*** p < 0.001).

    Article Snippet: Liver cancer stem cells (LCSCs) (sku: 36116-43; Celprogen, Torrance, CA, USA) and normal hepatocytes (NHs) (THLE-3; ATCC, Manassas, VA, USA) were cultured with Human Liver Cancer Stem Cell Media (Celprogen) and BEGM (Bronchial Epithelial Cell Growth Medium) (Lonza, Workingham, UK) using BEGM Bullet Kits (Lonza) at 37 °C, 5% CO 2 .

    Techniques: Control

    Inhibition of SATB2 expression suppresses cell viability in spheroids and stem cell markers and pluripotency maintaining factors in HepG2 and Hep3B cells. A, CSCs derived from primary HCC tissues were transduced with lentiviral particles expressing either SATB2 Crispr/Cas9 or non‐targeting control from a pooled population by targeting four different sites (GeneCopoeia, Rockville, MD). RNA was extracted from cells, and qRT‐PCR analysis was performed to measure the expression of SATB2. Data represent mean ± SD (n = 4). * = significantly different from NTC group ( P < .05). B, CSCs/NTC and CSCs/SATB2 Crispr/Cas9 cells were grown for 6 d, and cell viability in spheroids was measured by XTT assay. Data represent mean ± SD (n = 4). * = significantly different from NTC group ( P < .05). C and D, RNA was isolated from CSCs/NTC and CSCs/SATB2 Crispr/Cas9 cells. The qRT‐PCR analysis was performed to measure the expression of stem cell markers (CD133, CD44 and CD24) and pluripotency maintaining factors (c‐Myc, KLF4, SOX2 and OCT4). GAPDH was used as an internal control. Data represent mean ± SD (n = 4). * = significantly different from CSCs/NTC group, P < .05. Note that the expression of genes in the control group was normalized to 1

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Higher expression of SATB2 in hepatocellular carcinoma of African Americans determines more aggressive phenotypes than those of Caucasian Americans

    doi: 10.1111/jcmm.14652

    Figure Lengend Snippet: Inhibition of SATB2 expression suppresses cell viability in spheroids and stem cell markers and pluripotency maintaining factors in HepG2 and Hep3B cells. A, CSCs derived from primary HCC tissues were transduced with lentiviral particles expressing either SATB2 Crispr/Cas9 or non‐targeting control from a pooled population by targeting four different sites (GeneCopoeia, Rockville, MD). RNA was extracted from cells, and qRT‐PCR analysis was performed to measure the expression of SATB2. Data represent mean ± SD (n = 4). * = significantly different from NTC group ( P < .05). B, CSCs/NTC and CSCs/SATB2 Crispr/Cas9 cells were grown for 6 d, and cell viability in spheroids was measured by XTT assay. Data represent mean ± SD (n = 4). * = significantly different from NTC group ( P < .05). C and D, RNA was isolated from CSCs/NTC and CSCs/SATB2 Crispr/Cas9 cells. The qRT‐PCR analysis was performed to measure the expression of stem cell markers (CD133, CD44 and CD24) and pluripotency maintaining factors (c‐Myc, KLF4, SOX2 and OCT4). GAPDH was used as an internal control. Data represent mean ± SD (n = 4). * = significantly different from CSCs/NTC group, P < .05. Note that the expression of genes in the control group was normalized to 1

    Article Snippet: CD24+/CD44+/CD133+ human liver cancer stem cells (CSCs) were isolated from primary HCC (obtained from Celprogen) and grown in well‐defined stem cell culture medium as per the supplier's instructions.

    Techniques: Inhibition, Expressing, Derivative Assay, Transduction, CRISPR, Quantitative RT-PCR, XTT Assay, Isolation